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U6382

Anti-UCP-1 antibody produced in rabbit

Name: Anti-UCP-1 antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Ucp1 Antibody, Ucp1 Antibody - Anti-UCP-1 antibody produced in rabbit, Anti-Uncoupling Protein 1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01092514

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC (p), WB

Citations:

108

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Properties

biological source

rabbit

Quality Level

300

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~32 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of mouse brown adipose tissue., western blot: 1:1,000 using mitochondrial extract of rat brown adipose tissue

UniProt accession no.

P25874

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... UCP1(7350)
mouse ... Ucp1(22227)
rat ... Ucp1(24860)

Description

General description

Uncoupling protein (UCP1) is a 32 kDa, mitochondrial inner membrane protein. It is active as a proton channel forming dimer. It can bind purine nucleotides and is capable of being stimulated by fatty acids.

Immunogen

synthetic peptide corresponding to human UCP1 sequence (amino acids 145-159) with N-terminal cysteine added, conjugated to KLH. The corresponding sequence is identical in rat and mouse UCP1.

Application

Anti-UCP-1 antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Anti-UCP-1 specifically recognizes human, mouse and rat UCP-1. Staining of the UCP-1 band is specifically inhibited with the immunizing peptide. The epitopes recognized are compatible with routine formalin-fixation and paraffin-embedding.

Uncoupling proteins (UCPs) are transporters functioning as enzymatic uncouplers of oxidative phosphorylation. They can return protons pumped by the respiratory chain to the mitochondrial matrix. UCP-1 is exclusively expressed in brown adipose tissue (BAT) in rodents and in neonates where it is regulated by norepinephrine and thyroid hormones. UCP-1 has been shown to depend on CoQ (ubiquinone) as an obligatory co-factor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Information

低风险生物材料

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Global Trade Item Number

SKU	GTIN
U6382-100UL	04061838038067
U6382-.2ML	04061837409868
U6382-25UL	04061838448200