V8012
Anti-V5 antibody, Mouse monoclonal
clone V5-10, purified from hybridoma cell culture
Synonym(s):
V5 Tag Monoclonal Antibody, V5 antibody
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
V5-10, monoclonal
Application:
ICC, WB
Citations:
94
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
V5-10, monoclonal
concentration
~1 mg/mL
technique(s)
immunocytochemistry: 1-2 μg/mL, western blot: 0.5-1 μg/mL
shipped in
dry ice
storage temp.
−20°C
General description
Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide conjugated to KLH.
The mouse monoclonal Anti-V5 antibody reacts specifically with the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.
The mouse mooclonal Anti-V5 antibody reacts specifically with the V5-taged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.
Biochem/physiol Actions
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Instructions
Ernesto Ciabatti et al.
Cell, 170(2), 382-392 (2017-07-12)
Neural networks are emerging as the fundamental computational unit of the brain and it is becoming progressively clearer that network dysfunction is at the core of a number of psychiatric and neurodegenerative disorders. Yet, our ability to target specific networks
Blocking Double-Stranded RNA-Activated Protein Kinase PKR by Japanese Encephalitis Virus Nonstructural Protein 2A
Tu YC, et al.
Journal of Virology, 86(19), 10347-10358 (2012)
Sebastian Wissel et al.
G3 (Bethesda, Md.), 6(8), 2467-2478 (2016-06-10)
Traditional loss-of-function studies in Drosophila suffer from a number of shortcomings, including off-target effects in the case of RNA interference (RNAi) or the stochastic nature of mosaic clonal analysis. Here, we describe minimal in vivo GFP interference (miGFPi) as a
Global Trade Item Number
| SKU | GTIN |
|---|---|
| V8012-50UG | 04061837481383 |