XNAR
Extract-N-Amp™ Plant PCR Kit
sufficient for 1000 extractions, sufficient for 1000 amplifications
Synonym(s):
Plant direct PCR kit
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About This Item
NACRES:
NA.55
UNSPSC Code:
41106303
Quality Level
usage
sufficient for 1000 amplifications, sufficient for 1000 extractions
feature
dNTPs included, hotstart, Direct PCR
technique(s)
PCR: suitable
color
colorless
input
crude DNA
shipped in
wet ice
storage temp.
−20°C
General description
The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. The kit also includes a PCR ReadyMix™, especially formulated for amplification directly from extract.
Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR ReadyMix, included in the kit.
Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR ReadyMix, included in the kit.
The Extract-N-Amp™ Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.
Application
Extract-N-Amp™ Plant PCR Kit has been used for DNA extraction from fresh mushrooms.
Extract-N-Amp™ Plant PCR Kit is suitable for use in genotyping.
It has been used forDNA extraction from fresh mushrooms.
It has been used forDNA extraction from fresh mushrooms.
Features and Benefits
- Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
- A PCR ReadyMix™, specially formulated for amplification directly from extract
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- Extract stable at 4 °C for at least 6 months
- Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
- No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
- A specially formulated PCR ReadyMix is provided for use with the extracted DNA
- The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
- Suitable for high-throughput plant genetic analysis.
- Lengthy enzymatic digestions are not required, saving time and effort.
- The extracted DNA remains stable at 4 °C for atleast 6 months.
Other Notes
Extract-N-Amp™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Regulatory Information
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Protocols
The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.
Articles
简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.
Antoine Lf Gady et al.
Plant methods, 5, 13-13 (2009-10-09)
The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as
M Riahi et al.
Genetics and molecular research : GMR, 9(3), 1334-1342 (2010-07-21)
Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with
Dionne N Shepherd et al.
Journal of virological methods, 149(1), 97-102 (2008-02-19)
A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-Amp-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this method an attempt was made to isolate and
Global Trade Item Number
| SKU | GTIN |
|---|---|
| XNAR-1KT | 04061837549939 |