Y0503
Y-27632 dihydrochloride
synthetic (organic), ≥98% (HPLC), ROCK inhibitor, powder
Synonym(s):
Rock inhibitor y-27632, Y-27632 2HCl, (R)-(+)-trans-4-(1-Aminoethyl)-N-(4-Pyridyl)cyclohexanecarboxamide dihydrochloride
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About This Item
Empirical Formula (Hill Notation):
C14H21N3O · 2HCl
CAS Number:
Molecular Weight:
320.26
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.77
MDL number:
Product Name
Y-27632 dihydrochloride, ≥98% (HPLC)
biological source
synthetic (organic)
Quality Level
assay
≥98% (HPLC)
form
powder
optical activity
[α]/D +3.0 to +5.0°, c = 1.0 in methanol
color
white to beige
solubility
H2O: 14 mg/mL
storage temp.
2-8°C
SMILES string
Cl.Cl.C[C@@H](N)[C@H]1CC[C@@H](CC1)C(=O)Nc2ccncc2
InChI
1S/C14H21N3O.2ClH/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13;;/h6-12H,2-5,15H2,1H3,(H,16,17,18);2*1H/t10-,11-,12-;;/m1../s1
InChI key
IDDDVXIUIXWAGJ-LJDSMOQUSA-N
Gene Information
human ... ROCK1(6093), ROCK2(9475)
General description
Y-27632, a Rho-kinase inhibitor, counteracts noradrenergic contractions in the penile corpus cavernosum of rabbits and humans. It helps to relax vascular and non-vascular smooth muscle in various preparations and lower blood pressure in hypertensive rats. Additionally, this inhibitor induces reversible changes in cell shape and decreases in actin stress fibers, focal adhesions, and protein phosphotyrosine staining in human trabecular meshwork (TM) cells and Schlemm′s canal cells. Y-27632 inhibits the kinase activity of both ROCK-I and ROCK-II in vitro, and this inhibition is competitively reversed by ATP, indicating that these compounds bind to the catalytic site to inhibit the kinases. Ki = 140 nM for p160ROCK.
Y-27632 dihydrochloride is an effective bronchodilator and improves lung resistance (RL) induced by antigen and acetylcholine. Y-27632 controls neutrophil migration and lung edema and may be a potential agent against acute lung injury.The utilization of Y-27632 dihydrochloride, in conjunction with Accutase or other enzyme solutions, during the passaging of human embryonic stem (hES) cells has been demonstrated to enhance cell survival and significantly improve cloning efficiency
Application
Y-27632 dihydrochloride has been used:
- as a medium supplement in pancreatic ductal adenocarcinoma organoid culture
- in the inhibition of Ras homolog gene family (Rho) kinase in mouse embryonic stem cells
- in the inhibition of Rho-associated protein kinase (ROCK) in human embryonic stem cells and human induced pluripotent stem cells (iPSCs)
- Y-27632 dihydrochloride has been used as a supplement in the E8 medium to promote in vitro differentiation of human pluripotent stem cells
Biochem/physiol Actions
Y-27632 is a highly potent, cell-permeable, selective ROCK inhibitor.
Y-27632 is highly potent, cell-permeable, selective ROCK (Rho-associated coiled coil forming protein serine/threonine kinase) inhibitor. Ki = 140 nM for p160ROCK. Y-27632 also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP.
Features and Benefits
This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Legal Information
Sold under license from Mitsubishi Pharma Corporation
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Articles
可生成组织和干细胞来源3D脑、胃肠道、肺和癌症肿瘤类器官模型的类器官培养产品。
Discover Bioactive Small Molecules for Kinase Phosphatase Biology
Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
Basement membrane destruction by pancreatic stellate cells leads to local invasion in pancreatic ductal adenocarcinoma
Koikawa K, et al.
Cancer Letters, 425, 65-77 (2018)
Taisuke Kadoshima et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(50), 20284-20289 (2013-11-28)
Here, using further optimized 3D culture that allows highly selective induction and long-term growth of human ES cell (hESC)-derived cortical neuroepithelium, we demonstrate unique aspects of self-organization in human neocorticogenesis. Self-organized cortical tissue spontaneously forms a polarity along the dorsocaudal-ventrorostral
Naoki Okumura et al.
Investigative ophthalmology & visual science, 55(1), 318-329 (2013-10-10)
To investigate the molecular mechanism of Rho-associated kinase (ROCK) inhibitors Y-27632 and Y-39983 on corneal endothelial cell (CEC) proliferation and their wound-healing effect. The expression of G1 proteins of the cell cycle and expression of phosphorylated Akt in monkey CECs
Global Trade Item Number
| SKU | GTIN |
|---|---|
| Y0503-1MG | 04061835306190 |
| Y0503-5MG | 04061835306206 |