Anti-FOSL1 Antibody, clone 1D10 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Fos-related antigen 1 (UniProt: P15407; also known as FRA-1) is encoded by the FOSL1 (also known as FRA1) gene (Gene ID: 8061) in human. Fos-related antigen 1 (FOSL1) oncoprotein belongs to the activator protein-1 (AP-1) transcription factor family, which regulates gene expression involved in cell proliferation, differentiation, and survival. FOSL1 forms heterodimers with JUN (c-Jun, JunB, and JunD) proteins, mediating transcriptional activity. FOSL1 contains a basic leucine zipper (bZIP) domain that facilitates DNA binding and dimerization with JUN family members. Unlike c-Fos, FOSL1 lacks a transactivation domain, relying on its partners for transcriptional regulation. FOSL1 plays a critical role in immune responses, particularly in inflammatory pathways. It is involved in Th17 cell differentiation and autoimmune diseases such as rheumatoid arthritis through STAT3-mediated signaling. FOSL1 also modulates tumor progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis. microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) contribute to the regulation of FOSL1 expression by directly interacting with its mRNA transcripts. Overexpression of FRA1 has been observed in several cancers, including breast, lung, and pancreatic carcinomas, where it contributes to tumor invasiveness and resistance to apoptosis. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref: Khedri, A., et al., (2024), Int. J. Mol. Sci, 25(10); 5362).

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal half of human FOSL1.

Quality Control Testing

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected FOSL1 in L6 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected FOSL1 in U-87 MG cell, MDA-MB-231 cell, and NIH/3T3 cell lysates.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected FOSL1 in U-87 MG cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FOSL1 in human melanoma tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound with FOSL1 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.

Clone 1D10 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects FOSL1. It targets an epitope within 22 amino acids from the N-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.