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53334-U

Ascentis^® Express Phenyl-Hexyl (2.7 μm) HPLC Column

Name: Ascentis<SUP>®</SUP> Express Phenyl-Hexyl (2.7 μm) HPLC Column
Brand: SUPELCO

L × I.D. 5 cm × 2.1 mm, pk of 1 ea

Synonym(s):

Core-shell (SPP) Fused Core Phenyl HPLC column

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About This Item

UNSPSC Code:

41115700

NACRES:

SB.52

eCl@ss:

32110501

L × i.d.:

5 cm × 2.1 mm

Particle size:

2.7 μm

Matrix active group:

phenylhexyl phase

Pore size:

90 Å

Matrix:

Fused-Core particle platform, superficially porous particle

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Properties

material

stainless steel column

Quality Level

100

agency

suitable for USP L11

product line

Ascentis^®

feature

endcapped

packaging

pk of 1 ea

manufacturer/tradename

Ascentis^®

parameter

≤100 °C temp. range, 60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

5 cm × 2.1 mm

surface area

135 m²/g

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

phenylhexyl phase

particle size

2.7 μm

pore size

90 Å

pH range

2-9

application(s)

food and beverages

separation technique

reversed phase

Description

General description

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

Application

A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers: Demonstrates the robustness of the Ascentis^® Express Phenyl-Hexyl, 2.7 μm HPLC column in automating the detection of contaminants in beverages. This study showcases the column′s capacity for high throughput analysis, essential for quality control in the food industry, ensuring safety and compliance with regulatory standards (Lhotská et al., 2016).

Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids: This research highlights the efficiency of the Ascentis^® Express Phenyl-Hexyl column in analyzing complex phenolic compounds, illustrating its superior performance in environmental and pharmaceutical applications. The column′s design enables enhanced separation capabilities, crucial for accurate phenolic profiling in various samples (Chocholouš et al., 2013).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

LC/MS/MS Analysis of Aflatoxins in Cannabis on Ascentis® Express 2.7µm Phenyl-Hexyl after Cleanup Using Supel™ Tox AflaZea SPE

solution, 2 μg/mL in acetonitrile, analytical standard

LC/MS/MS Analysis of Aflatoxins in Hops on Ascentis® Express 2.7 μm Phenyl-Hexyl after Cleanup Using Supel™ Tox AflaZea SPE

Online SPE/LC-MS Method for the Rapid Analysis of Thyroid Hormones in Plasma

Determination of the thyroid hormones T4, T3 and rT3 from plasma using an Ascentis Express RP-Amide cartridge to trap the analytes, and a Phenyl-Hexyl column to resolve them.

Articles

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

Quick, Sensitive LC/MS/MS Analysis of Aflatoxins in Cannabis

Analyte recovery values from spiked cannabis extracts ranged from 102 to 127% with RSD below 12% for 3 replicates. Excellent recovery values were even observed for Aflatoxins B2 and G2 which were spiked at a low level of 6.1 ppb.

Global Trade Item Number

SKU	GTIN
53334-U	04061838530646