53341-U
Ascentis® Express Phenyl-Hexyl (2.7 μm) HPLC Column
L × I.D. 3 cm × 3 mm, pk of 1 ea
Synonym(s):
Core-shell (SPP) Fused Core Phenyl HPLC column
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About This Item
UNSPSC Code:
41115700
NACRES:
SB.52
eCl@ss:
32110501
L × i.d.:
3 cm × 3 mm
Particle size:
2.7 μm
Matrix active group:
phenylhexyl phase
Pore size:
90 Å
Matrix:
Fused-Core particle platform, superficially porous particle
material
stainless steel column
Quality Level
agency
suitable for USP L11
product line
Ascentis®
feature
endcapped
packaging
pk of 1 ea
manufacturer/tradename
Ascentis®
parameter
60 °C temp. range, 600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
3 cm × 3 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform, superficially porous particle
matrix active group
phenylhexyl phase
particle size
2.7 μm
pore size
90 Å
pH range
2-9
application(s)
food and beverages
separation technique
reversed phase
General description
The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.
Application
- A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers: Demonstrates the robustness of the Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC column in automating the detection of contaminants in beverages. This study showcases the column′s capacity for high throughput analysis, essential for quality control in the food industry, ensuring safety and compliance with regulatory standards (Lhotská et al., 2016).
- Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids: This research highlights the efficiency of the Ascentis® Express Phenyl-Hexyl column in analyzing complex phenolic compounds, illustrating its superior performance in environmental and pharmaceutical applications. The column′s design enables enhanced separation capabilities, crucial for accurate phenolic profiling in various samples (Chocholouš et al., 2013).
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Articles
Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.
C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 53341-U | 04061838530684 |