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53345-U

Ascentis® Express Phenyl-Hexyl (2.7 μm) HPLC Column

L × I.D. 10 cm × 3 mm, pk of 1 ea

Synonym(s):

Core-shell (SPP) Fused Core Phenyl HPLC column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
eCl@ss:
32110501
L × i.d.:
10 cm × 3 mm
Particle size:
2.7 μm
Matrix active group:
phenylhexyl phase
Pore size:
90 Å
Matrix:
Fused-Core particle platform, superficially porous particle
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material

stainless steel column

Quality Level

100

agency

suitable for USP L11

product line

Ascentis®

feature

endcapped

packaging

pk of 1 ea

manufacturer/tradename

Ascentis®

parameter

60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

10 cm × 3 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

phenylhexyl phase

particle size

2.7 μm

pore size

90 Å

pH range

2-9

application(s)

food and beverages

separation technique

reversed phase

General description

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

Application


  • A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers.: This study presents a rapid, automated method for the detection of mycotoxins in beer using high-performance liquid chromatography. The use of the Ascentis® Express Phenyl-Hexyl column enables efficient and effective sample processing, demonstrating its applicability in food safety and quality control (Lhotska et al., 2016).

  • Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids.: This paper explores the benefits of using core-shell particle technology, including Ascentis® Express Phenyl-Hexyl columns, in the analysis of phenolic acids. The study highlights the columns′ enhanced performance and efficiency, making them valuable for chemical analysis in environmental and food samples (Chocholous et al., 2013).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
USDW002358
USDW002357
USDW002356
USDW002355
USDW002354

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Articles

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

Teriparatide Peptide Mapping Analysis by LC-UV-MS

LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
View All Related Papers

Global Trade Item Number

SKUGTIN
53345-U04061838530714