Key Documents

View All Documentation

66905-U

BIOshell^™ A160 Peptide C18 (2.7 μm) HPLC Columns

Name: BIOshell<SUP>&#8482;</SUP> A160 Peptide C18 (2.7 μm) HPLC Columns
Brand: SUPELCO

L × I.D. 15 cm × 2.1 mm, HPLC Column

Synonym(s):

Superficially Porous Wide Pore C18 UHPLC Column

Select a Size

Change View

About This Item

UNSPSC Code:

41115700

NACRES:

SB.52

L × i.d.:

15 cm × 2.1 mm

Particle size:

2.7 μm

Matrix active group:

C18 (octadecyl) bonding phase, diisobutyloctadecyl

Pore size:

160 Å

Matrix:

spherical silica particle platform, superficially porous particle

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

BIOshell^™ A160 Peptide C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

material

stainless steel hardware

Quality Level

100

agency

suitable for USP L1

description

Shell thickness (0.5 μm), Solid Core (1.7 μm)

product line

BIOshell^™

feature

endcapped: no

analyte chemical class(es)

peptides

manufacturer/tradename

BIOshell^™

packaging

1 ea of

extent of labeling

4.6% carbon loading

parameter

600 bar max. pressure, 90 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

15 cm × 2.1 mm

surface area

90 m²/g

surface coverage

2.2 μmol/m²

matrix

spherical silica particle platform, superficially porous particle

matrix active group

C18 (octadecyl) bonding phase, diisobutyloctadecyl

particle size

2.7 μm

pore size

160 Å

operating pH range

1-8

separation technique

reversed phase

Description

General description

BIOshell^™ 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected C18 ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C). This greatly expands the application range for the separation of biomolecules.

Application

BIOshell^™ A160 Peptide C18, 2.7 μm HPLC Column, a non-end capped column that has superficially porous particle with C18 alkyl ligand as the stationary phase, is used mainly in the separation of peptides at a good flow rate.

UHPLC Analysis of Peptides on BIOshell^™ A160 Peptide C18, CN, and Phenyl-Hexyl, 2.7 μm

Features and Benefits

BIOshell^™ A160 Peptide C18, 2.7 μm HPLC Columns are compatible both with UHPLC and HPLC systems. They are specifically engineered for the fast and high-resolution seperation of biomolecules. These columns operate at high flow rates and have 40% higher efficiency than columns with fully porous particles of the same size. They are stable at high temperatures, which increases the throughput and enhances the peak shape.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Still not finding the right product?

Explore all of our products under BIOshell A160 Peptide C18, 2.7 μm HPLC Column

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

HPLC Analysis of Insulin Variants and Analogs

Chromatographic technique identifies insulin variants efficiently, aiding in pharmacokinetics.

Articles

Ascentis^® Express Peptide ES-C18 U/HPLC Columns

Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.

Protein Fingerprinting of a Viral Vector, AAV5

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

Teriparatide Peptide Mapping Analysis by LC-UV-MS

LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

View All Articles

Collagen-derived X-Hyp-Gly-type tripeptides promote differentiation of MC3T3-E1 pre-osteoblasts

Taga Y, et al.

Journal of functional foods, 46, 456-462 (2018)

Global Trade Item Number

SKU	GTIN
66905-U	04061833354650