V900483
Trizma® base
≥99%, crystalline powder, reagent grade, Vetec™
Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
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About This Item
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
UNSPSC Code:
12161700
PubChem Substance ID:
EC Number:
201-064-4
Beilstein/REAXYS Number:
741883
MDL number:
Product Name
Trizma® base, Vetec™, reagent grade, ≥99%
grade
reagent grade
description
aminopeptidase substrate
product line
Vetec™
assay
≥99%
form
crystalline powder
pH
10.5-12
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
solubility
water: 0.667 g/mL, clear, colorless to faintly yellow
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
General description
Trizma® base is a basimetric standard used in biochemistry and molecular biology. It may be used as a buffer or as a component of mixed buffer formulations. It is essentially stable, relatively non-hygroscopic and has a high equivalent weight.
Application
Trizma® base can be used as a buffer:
- In the evanescent absorption analysis of NADPH.
- In the autoxidation studies of oxymyoglobin.
Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Preparation of buffers for use in enzyme studies
Handbook of Biochemistry and Molecular Biology (2010)
Autoxidation of native oxymyoglobin. Kinetic analysis of the pH profile
Shikama K and Sugawara Y
European Journal of Biochemistry, 91(2), 407-413 (1978)
Tapered fibers for cell studies
Reviews in Fluorescence (2005)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| V900483-1KG | 04061826565124 |
| V900483-500G | 04061833495155 |