D7291
脱氧核糖核酸酶 I 无 RNase 溶液 来源于牛胰腺
别名:
DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.53
EC Number:
232-667-0
MDL number:
Specific activity:
10000 units/mg protein
Biological source:
bovine pancreas
Concentration:
5—15 mg protein/mL
biological source
bovine pancreas
Quality Level
grade
Molecular Biology
form
buffered aqueous glycerol solution
specific activity
10000 units/mg protein
mol wt
29.1 kDa
concentration
5—15 mg protein/mL
technique(s)
DNA extraction: suitable
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
foreign activity
RNase, none detected
storage temp.
−20°C
Gene Information
cow ... DNASE1(282217)
General description
脱氧核糖核酸酶Ⅰ将单链和双链 DNA 消化为带 5′磷酸盐和3′羟基末端的单核苷酸和寡核苷酸混合物。这种催化活性依赖于二价离子。在存在Mg2+的情况下，脱氧核糖核酸酶Ⅰ随机而独立地水解双链DNA的每条链。在存在Mn2+的情况下，两条链都可以被切开。
Application
来自牛胰脏的无RNA酶脱氧核糖核酸酶I溶液已被用于消化各种样品中的DNA。
在分子生物学应用中用于RNA纯化过程中去除DNA，用于制备DNA进行缺口翻译，以及通过足迹法分析DNA-蛋白质相互作用。
Other Notes
DNase I 溶于 50% (v/v) 甘油、20 mM 醋酸钠 (pH 6.5)、5 mM CaCl2 和 0.1 mM PMSF 溶液中。
使用小牛胸腺DNA作为底物时，一单位在每毫升反应混合物中每分钟可引起0.001的ΔA260。
用这种DNase I处理过的RNA不应该用于生成cDNA文库或用于RT-PCR。对于这些敏感的应用，建议使用扩展级DNase I，目录号AMP-D1。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
动植物源性产品
此项目有
实验方案
Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.
商品
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
A Deoxyribonuclease from Chlamydomonas reinhardii:Purification and Properties
TAIT GCL and HARRIS WJ
European Journal of Biochemistry, 75(2), 357-364 (1977)
Site-specific protein-DNA photocrosslinking. Analysis of bacterial transcription initiation complexes.
N Naryshkin et al.
Methods in molecular biology (Clifton, N.J.), 148, 337-361 (2001-05-19)
Stage-Specific Expression Profiles of Anti-Mullerian Hormone and its Type II Receptor in Germ Cells during Spermatogenic Cycle of Rats
Ohyama K, et al.
Journal of Steroids & Hormonal Science, 8(187), 2-2 (2017)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D7291-.5MG | 04061833588376 |
| D7291-2MG | 04061833588383 |