Quality Level
technique(s)
whole genome amplification: suitable
dilution
(WTA)
input
purified DNA
shipped in
wet ice
storage temp.
−20°C
General description
TransPlex®，一种全转录组扩增（WTA）方法，能够在4小时内，代表性地扩增出纳克级的总RNA而没有3′-偏差。 从组织、培养细胞、福尔马林固定样品或血清中所获得的微克级扩增产物适用于例如qPCR和微阵列分析等下游应用。
Application
TransPlex®全转录组扩增试剂盒已用于合成双链cDNA。还可用于cDNA扩增。
适于各种下游应用，包括：
- qPCR
- 微阵列分析
- 克隆
Biochem/physiol Actions
WTA过程包括2个步骤。首先，使用由拟随机3′端和通用5′端组成的非自身互补引物对样品RNA进行逆转录。随着聚合作用的发生，被取代的单链将作为引物退火和延伸的新模板。所得OmniPlex cDNA文库由以通用末端序列为侧翼序列、含100-1000个碱基的随机重叠片段组成。随后，使用通用引物对文库进行PCR扩增，生成WTA产物。
Features and Benefits
- 在4小时内对总RNA进行扩增，手动操作时间低于30分钟
- 仅需5 ng起始材料，即可从总RNA扩增为极具代表性的文库
- 以组织、培养的细胞、血清或来自福尔马林固定的石蜡包埋样品的降解RNA为原料，从完整的RNA可生成微克级的扩增产物
Legal Information
TransPlex is a registered trademark of Rubicon Genomics, Inc.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
存储类别
12 - Non Combustible Liquids
法规信息
常规特殊物品
此项目有
实验方案
TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.
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Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| WTA1-50RXN | 04061835378807 |