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B6650

X-GlcA

Name: X-GlcA
Brand: SIGMA

reagent for selection of recombinant bacterial clones

别名:

5-溴-4-氯-3-吲哚基 β-D-葡糖醛酸环己铵盐, X-GlcA, X-葡糖苷酸 CHA 盐

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关于此项目

经验公式（希尔记法）:

C₁₄H₁₃BrClNO₇ · C₆H₁₃N

化学文摘社编号:

114162-64-0

分子量:

521.79

NACRES:

NA.85

PubChem Substance ID:

24891926

UNSPSC Code:

12352200

MDL number:

MFCD00058543

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属性

grade

Molecular Biology

Quality Level

200

sterility

non-sterile

assay

≥98% (TLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for β-galactosidase test

storage temp.

−20°C

SMILES string

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

InChI

1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1

InChI key

JXCKZXHCJOVIAV-CYRSAHDMSA-N

说明

General description

X-GlcA 是 β-半乳糖苷酶的显色底物，用于确定琼脂平板上生长的细菌中是否存在克隆的 DNA 插入片段。 X-GlcA 设计用于具有 lac+ 表型的重组细菌菌落的蓝白色选择中替代 X-Gal。

Application

适用于选择具有 lac⁺ 表型的重组细菌菌落。
X-GlcA 已用于根切片的组化染色，用于光学显微镜观察。

β-葡萄糖醛酸酶 (GUS) 基因检测的显色底物。

Biochem/physiol Actions

当 X-GlcA 被 β-半乳糖苷酶水解时，所得产物将形成蓝色沉淀。在 X-GlcA 存在下生长的 Lac+ 菌落会变成深蓝色，从而易于区分 lac+ 和 lac- 菌落。

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