产品名称
杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
Application
Dulbecco′s 改良Eagles 培养基- 高葡萄糖已被用于各种细胞类型的培养基,包括人宫颈内上皮End1/E6E7细胞、人腺癌上皮细胞(HeLa)、人肺泡腺癌上皮细胞(A549)、人骨肉瘤上皮细胞、人二倍体细胞系、原代肝细胞和滑膜组织
Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。
Preparation Note
按照每升培养基13.4克粉末配制。
补充3.7g/L碳酸氢钠。
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性,应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
用于传代和分装悬浮细胞系的细胞培养方案
Cell culture protocol for passaging and splitting suspension cell lines
相关内容
Instructions
Ana M S Cardoso et al.
Human molecular genetics, 28(16), 2738-2751 (2019-05-16)
Glioblastoma (GB) is the most aggressive and common form of primary brain tumor, characterized by fast proliferation, high invasion, and resistance to current standard treatment. The average survival rate post-diagnosis is only of 14.6 months, despite the aggressive standard post-surgery treatment
Lingling Lu et al.
Acta histochemica, 121(6), 695-703 (2019-06-27)
Renal fibrosis is a common mechanism that leads to all kidney diseases and Epithelial-mesenchymal transition (EMT) is considered as one of the potential mechanisms of renal fibrosis. Inhibitor of growth 4 (ING4) was reported to involve in several diseases; especially
Ayako Sasaki et al.
Human cell, 29(1), 37-45 (2015-11-11)
Of two stereoisomers of glucose, only D- and not L-glucose is abundantly found in nature, being utilized as an essential fuel by most organisms. The uptake of D-glucose into mammalian cells occurs through glucose transporters such as GLUTs, and this
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D5648-10L | 04061833586532 |
| D5648-25KG | 04061834408376 |
| D5648-10X1L | 04061835518951 |
| D5648-50L | 04061833586549 |