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Merck
CN

D5648

DMEM - high glucose

With ʟ-glutamine, without sodium bicarbonate and sodium pyruvate, powder, suitable for cell culture

别名:

DME, DMEM

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
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产品名称

杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco′s 改良Eagles 培养基- 高葡萄糖已被用于各种细胞类型的培养基,包括人宫颈内上皮End1/E6E7细胞、人腺癌上皮细胞(HeLa)、人肺泡腺癌上皮细胞(A549)、人骨肉瘤上皮细胞、人二倍体细胞系、原代肝细胞和滑膜组织
Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。

Preparation Note

按照每升培养基13.4克粉末配制。
补充3.7g/L碳酸氢钠。


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存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

粉末培养基及盐混合物极具吸湿性,应注意防潮。

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

商品

用于传代和分装悬浮细胞系的细胞培养方案

Cell culture protocol for passaging and splitting suspension cell lines

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全球贸易项目编号

货号GTIN
D5648-10L04061833586532
D5648-25KG04061834408376
D5648-10X1L04061835518951
D5648-50L04061833586549