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Merck
CN

L8902

来自 菜豆的凝集素 (红芸豆)

Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

别名:

PHA, 植物血球凝集素(菜豆)

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关于此项目

UNSPSC Code:
12352202
EC Number:
232-718-7
NACRES:
NA.32
Form:
lyophilized powder
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Quality Level

product line

BioReagent

form

lyophilized powder

potency

<40 μg per mL agglutination activity

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

solubility

PBS: 2 mg/mL

storage temp.

2-8°C

Application

来自菜豆(Phaseolus vulgaris) (红芸豆)的凝集素可用于淋巴细胞转化试验和培养基。

Biochem/physiol Actions

PHA由两种分子种类组成,即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素(PHA-E),和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素(PHA-L)。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。

Analysis Note

凝集活性以μg/mL表示,并由1mg/ml溶液的连续稀释液进行测定。该活性是在25℃温育1小时后,在pH6.8的磷酸盐缓冲盐水中,凝集2%人红细胞悬浮液所需的最低浓度。


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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料

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历史批次信息供参考:

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L8902-100MG04061833966228
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