Quality Level
usage
kit sufficient for 10 extractions (1 ml packed cell volume), kit sufficient for 100 extractions (100 μl packed cell volume)
technique(s)
protein extraction: suitable, western blot: suitable
shipped in
dry ice
storage temp.
−20°C
General description
其中的核内蛋白提取方法步骤可使细胞在低渗缓冲液中膨胀。随后细胞破碎，细胞质成分去除，核内蛋白质在高盐缓冲液条件下释放出来。
Application
NXTRACT试剂盒用于研究盐对降压性盐皮质激素治疗小鼠心脏差异基因表达和冠状动脉病变的影响。它还用于测试穿心莲内酯在治疗子宫内膜异位症方面的潜在作用。
Other Notes
此试剂盒包含一套从哺乳动物组织或培养细胞制备细胞核和细胞质蛋白提取物的完整系统。所有提取所需的试剂均包含在内。
通过详细技术公告中的大量操作流程，可实现特定应用最适合的选择。 例如，可选择含去垢剂和不含去垢剂的核蛋白提取程序，或者适用于大多数细胞类型的标准低渗裂解缓冲液及适用于脆弱细胞的等渗裂解缓冲液。 此外，该试剂盒还提供了通过稀释缓冲液从核提取物中减少盐分的操作流程。 NuCLEAR可灵活满足您的理想蛋白提取需求。 可在2小时内制出提取物，且由于核提取物与细胞质提取物之间几乎不存在交叉污染，因此纯度很高。
Legal Information
NuCLEAR is a trademark of Sigma-Aldrich Co. LLC
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产品编号
说明
化学品安全说明书 & 价格
signalword
Danger
存储类别
8A - Combustible corrosive hazardous materials
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
wgk
WGK 3
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
法规信息
常规特殊物品
低风险生物材料
此项目有
商品
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
K A Lee et al.
Gene analysis techniques, 5(2), 22-31 (1988-03-01)
A convenient and rapid method for preparing soluble extracts from the nuclei of as few as 3 x 10(7) mammalian cells (miniextract procedure) is described. By several criteria, miniextracts are comparable to nuclear extracts prepared from large numbers of cells
Isolation of intact nuclei for nuclear extract preparation from a fragile B-lymphocyte cell line.
R B Dyer et al.
BioTechniques, 19(2), 192-195 (1995-08-01)
Fangyuan Dong et al.
EBioMedicine, 39, 472-483 (2018-12-12)
Accumulating evidence has revealed the pivotal role of epigenetic regulation in the pathogenesis of liver disease. However, the epigenetic mechanism that accounts for hepatic stellate cells (HSCs) activation in liver fibrosis remains largely unknown. Primary HSCs were used to screen
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| NXTRACT-1KT | 04061838256133 |