P5747
单克隆抗-磷酸丝氨酸 小鼠抗
clone PSR-45, purified from hybridoma cell culture
别名:
单克隆抗磷酸丝氨酸, 磷酸化丝氨酸
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
PSR-45, monoclonal
Application:
ARR, DB, ELISA (i), WB
Citations:
34
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PSR-45, monoclonal
form
buffered aqueous solution
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
dot blot: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, microarray: suitable, western blot: 2.5-5.0 μg/mL using total rat brain extract.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Immunogen
磷酸丝氨酸与钉形贝血蓝蛋白（KLH）结合。
Biochem/physiol Actions
蛋白质磷酸化和脱磷酸化是改变真核细胞中蛋白质功能的基本信号传导机制。在正常组织中，磷酸化是一种罕见的遗传翻译后活动，但是磷酸化细胞蛋白质的丰度在多个激活过程后增加了数倍。被磷酸化的主要氨基酸是酪氨酸、丝氨酸或苏氨酸（pTyr/pSer/pThr），且每种氨基酸都有对其磷酸化的特异性激酶和对其脱磷酸化的特异性磷酸酶。针对于磷酸化残基的单克隆和多克隆抗体已经生成，并可用于分析和制备工具进行磷酸化细胞蛋白的鉴定、定量和免疫亲和分离。
通过 ELISA 和斑点印迹，该抗体以游离氨基酸形式或与载体（如 BSA 或 KLH）的缀合，与磷酸化丝氨酸特异性反应。与非磷酸化丝氨酸、磷酸苏氨酸、磷酸酪氨酸、AMP 或 ATP 无交叉反应。该抗体已用在免疫印迹中定位一些含磷酸丝氨酸蛋白质。由于识别位点的空间位阻，该抗体可能无法识别已知含有磷酸化丝氨酸的某些蛋白质。
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
法规信息
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低风险生物材料
此项目有
Matteo Fornai et al.
The Journal of pharmacology and experimental therapeutics, 356(2), 434-444 (2015-11-20)
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M Zhao et al.
FEBS letters, 509(3), 405-412 (2001-12-26)
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P R Murphy et al.
Endocrinology, 142(1), 81-88 (2001-01-06)
We recently reported that the rat Nb2 T lymphoma cells expressed messenger RNAs (mRNAs) encoding both fibroblast growth factor-2 (FGF-2) and the FGF receptor, suggesting possible paracrine and/or autocrine roles for FGF-2 in lymphoma cell function. We have also shown
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P5747-100UL | 04061838032461 |
| P5747-200UL | 04061834392323 |
| P5747-25UL | 04061838454065 |