form
solution
Quality Level
usage
sufficient for ≥600 reactions
feature
PCR Additives
concentration
100 ×
technique(s)
PCR: suitable
color
red
input
crude DNA
solubility
water: soluble
storage temp.
2-8°C
General description
Sigma′定量聚合酶链反应（qPCR）参考染料是一种专有染料，用于实时PCR。在使用SYBR绿、分子探针或双标记探针化学进行实时检测时，它可以用于反应数据的标准化。 我们提供100×参考染料，最大激发和放射峰分别出现在586nm和605nm。ROX参考染料仪器设定符合qPCR参考染料检测。
Application
定量 PCR 参考染料已被用于:
- 制备用于实时定量聚合酶链反应 （RT-qPCR）的预混液
- 作为反应混合物的成分，用于通过定量聚合酶链式反应（qPCR）检测艰难梭菌
- 通过 qPCR 分析细胞 DNA 污染程度
Other Notes
定量PCR参考染料仅用于研发目的。不可用于药物、家庭或其他用途。
signalword
Warning
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
ppe
Eyeshields, Gloves
实验方案
我们的 SYBR Green qPCR 协议是专为检测基因表达和 RT-PCR 反应的精确定量而设计的方法。
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
商品
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Increased environmental sample area and recovery of Clostridium difficile spores from hospital surfaces by quantitative PCR and enrichment culture
Brown KA, et al.
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R4526-.3ML | 04061836695354 |
| R4526-20RXN | 04061832933283 |
| R4526-5ML | 04061837693939 |