T1063
Thrombin 来源于人类血浆
lyophilized powder, ≥2800 NIH units/mg protein (E1%/280, 18.3)
别名:
IIa因子
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
Specific activity:
≥2800 NIH units/mg protein (E1%/280, 18.3)
General description
凝血酶是止血过程中的最终凝血蛋白酶，可同时促进促凝血和抗凝血作用。
Application
凝血酶被用于重组融合蛋白的位点特异性切割，这些重组融合蛋白具有可接近的凝血酶识别位点以除去亲和标签。凝血酶已可用于评估低分子量肝素在 体外 对肝硬化患者在肝病不同阶段的凝血酶生成的影响。
Biochem/physiol Actions
选择性裂解纤维蛋白原中 Arg-Gly 键形成纤维蛋白和纤维蛋白肽 A 和 B 的丝氨酸蛋白酶。
Physical form
冻干品来自于0.02 M Bis/Tris缓冲液，pH 6.5，含0.15M氯化钠和0.1% PEG-8000
Analysis Note
NIH 试验步骤基于 Biggs 方法的改进，使用 0.2mL 稀释血浆（与生理盐水 1:1 混合，作为底物）和 0.1mL Biggs 凝血酶样品（稳定于 1% 缓冲白蛋白溶液中）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
Other Notes
查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
活性以NIH单位表示，它是通过与NIH凝血酶参考标准品直接对比而获得的
Disclaimer
供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
高风险级别生物产品--人源产品
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商品
凝血酶因子IIa是一种内切丝氨酸蛋白酶，可选择性地裂解纤维蛋白原的Arg -Gly键，形成纤维蛋白并释放纤维蛋白肽A和B。
Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
M Senzolo et al.
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Massimo D'Agostino et al.
Nature, 551(7682), 634-638 (2017-11-02)
Membrane fusion in eukaryotic cells mediates the biogenesis of organelles, vesicular traffic between them, and exo- and endocytosis of important signalling molecules, such as hormones and neurotransmitters. Distinct tasks in intracellular membrane fusion have been assigned to conserved protein systems.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T1063-1KU | 04061837336478 |
| T1063-250UN | 04061837336485 |