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T7513

凝血酶 来源于牛血浆

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

别名:

IIa 因子

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关于此项目

化学文摘社编号:
9002-04-4
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
MFCD00166053
EC 号:
3.4.21.5 (BRENDA, IUBMB)
Specific activity:
≥2,000 NIH units/mg protein (E1%/280 = 19.5)
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form

lyophilized powder

Quality Level

200

specific activity

≥2,000 NIH units/mg protein (E1%/280 = 19.5)

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

UniProt accession no.

P00735

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

cow ... F2(280685)

Biochem/physiol Actions

丝氨酸蛋白酶，选择性断开纤维蛋白原中的精氨酸-甘氨酸键，形成纤维蛋白以及纤维蛋白肽 A 和 B。

Physical form

从生理盐水柠檬酸钠缓冲液（pH 6.5）冻干

Analysis Note

基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆（由血浆与等量生理盐水混合制得）作为底物，以及 0.1mL 凝血酶样品（在含 1% 白蛋白缓冲液中保持稳定）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
活性是通过与一种K批次的NIH凝血酶参考标准品进行直接比较而得到并以NIH单位进行表达的。

Other Notes

查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
活性以NIH单位表示，通过与NIH凝血酶参考标准品直接对比确定。

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCL5520
SLCK6302
SLCJ7952
SLCJ0343
SLCH4980

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实验方案

牛血浆凝血酶

凝血酶是一种内溶性丝氨酸蛋白酶，可选择性裂解纤维蛋白原的精氨酸-甘氨酸键，从而形成纤维蛋白并释放纤维蛋白肽A和B。

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

商品

凝血酶因子IIa

凝血酶因子IIa是一种内切丝氨酸蛋白酶，可选择性地裂解纤维蛋白原的Arg -Gly键，形成纤维蛋白并释放纤维蛋白肽A和B。

Thrombin Factor IIa

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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全球贸易项目编号

货号GTIN
T7513-250UN04061835559350
T7513-500UN04061835559367
T7513-1000KU04061831169485
T7513-100UN04061835546435
T7513-50UN04061837373510