B6429
Casein Blocking Buffer 10x
for Northern and Southern blotting, solution
Synonym(s):
10x Blocking Buffer
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About This Item
NACRES:
NA.54
UNSPSC Code:
12161700
grade
Molecular Biology
Quality Level
form
solution
technique(s)
Southern blotting: suitable
storage temp.
room temp
General description
Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
Application
Casein Blocking Buffer 10x has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
Features and Benefits
- Better blocking performance than dried milk or BSA
- Easy-to-use concentrate
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent and colorimetric detection assays
Preparation Note
Dilute 10x Blocking Buffer with molecular biology grade water before use.
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
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Protocols
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| B6429-500ML | 04061833434796 |