913839
2-Methylnicotinic acid imidazolide
Synonym(s):
(1H-imidazol-1-yl)(2-methylpyridin-3-yl)methanone, 1H-Imidazol-1-yl(2-methyl-3-pyridinyl)methanone, 3-(1H-imidazole-1-carbonyl)-2-methylpyridine, NAI, RNA SHAPE probe, RNA structure probe, mNAI
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About This Item
Empirical Formula (Hill Notation):
C10H9N3O
CAS Number:
Molecular Weight:
187.20
UNSPSC Code:
12352106
NACRES:
NA.22
MDL number:
Form:
powder
form
powder
Quality Level
mp
68-72 °C
storage temp.
2-8°C
SMILES string
[n]2(cncc2)C(=O)c1c(nccc1)C
InChI
1S/C10H9N3O/c1-8-9(3-2-4-12-8)10(14)13-6-5-11-7-13/h2-7H,1H3
InChI key
OWQPEDNXDCVXJO-UHFFFAOYSA-N
Application
2-Methylnicotinic acid imidazolide (NAI or mNAI) is an RNA SHAPE probe for live-cell RNA structure profiling. SHAPE -- or selective 2′-hydroxyl acylation analyzed by primer extension -- uses small, electrophilic chemical probes to react with the 2′-hydroxyl group and provides insight to RNA structure and its dynamics in varied cells states or in cells with gene knockouts. It has also been extended to the SHALiPE approach, selective 2′-hydroxyl acylation with lithium ion-based primer extension.
Other Notes
Conditional control of RNA-guided nucleic acid cleavage and gene editing
Guidelines for SHAPE Reagent Choice and Detection Strategy for RNA Structure Probing Studies
SnapShot: RNA Structure Probing Technologies
Structural Analysis using SHALiPE to Reveal RNA G-Quadruplex Formation in Human Precursor MicroRNA
Determination of in vivo RNA structure in low-abundance transcripts
Guidelines for SHAPE Reagent Choice and Detection Strategy for RNA Structure Probing Studies
SnapShot: RNA Structure Probing Technologies
Structural Analysis using SHALiPE to Reveal RNA G-Quadruplex Formation in Human Precursor MicroRNA
Determination of in vivo RNA structure in low-abundance transcripts
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Chun Kit Kwok et al.
Nature communications, 4, 2971-2971 (2013-12-18)
RNA structure plays important roles in diverse biological processes. However, the structures of all but the few most abundant RNAs are presently unknown in vivo. Here we introduce DMS/SHAPE-LMPCR to query the in vivo structures of low-abundance transcripts. DMS/SHAPE-LMPCR achieves
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 913839-500MG | 04061842050758 |
| 913839-50MG | 04061842050765 |