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Merck
CN

77515

Methylene Blue solution

suitable for microscopy

Synonym(s):

Ehrlich’s reagent III, Loeffler’s Methylene Blue

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
Molecular Weight:
319.85
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3641570
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form

solution

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria

suitability

suitable for microscopy, Corynebacterium spp., bacteria, mycobacteria, spore forming microorganisms

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

Application

Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm.

Other Notes

Composition:
methylene blue 0.3 g
ethanol 30 ml
water 100 ml


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pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 2

flash_point_f

113.0 °F

flash_point_c

45 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

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Nicolaas Lucius Gerlach et al.
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 72(4), 666-671 (2014-02-01)
To establish the accuracy of cone-beam computed tomographic (CBCT) views in determining the position and diameter of the mandibular canal. Two freshly frozen cadaver heads, 1 dentate and 1 edentate, were used to acquire CBCT scans. Measurements on cross-sectional CBCT
Q R J G Tummers et al.
European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 40(7), 850-858 (2014-05-28)
Despite recent developments in preoperative breast cancer imaging, intraoperative localization of tumor tissue can be challenging, resulting in tumor-positive resection margins during breast conserving surgery. Based on certain physicochemical similarities between Technetium((99m)Tc)-sestamibi (MIBI), an SPECT radiodiagnostic with a sensitivity of
Xiaojuan Bai et al.
Langmuir : the ACS journal of surfaces and colloids, 29(9), 3097-3105 (2013-02-20)
ZnO1-x/graphene hybrid photocatalyst was prepared via a facile in-situ reduction of graphene oxide and ZnO1-x surface defect oxide. The hybrid photocatlayst showed enhanced photocatalytic activity for the photodegradation of methylene blue. The photocorrosion of ZnO1-x was successfully inhibited by graphene