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93572

Formamide

puriss. p.a., ACS reagent, ≥99.5% (GC/T)

Synonym(s):

Amide C1, Formic amide

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About This Item

Linear Formula:
HCONH2
CAS Number:
75-12-7
Molecular Weight:
45.04
EC Number:
200-842-0
UNSPSC Code:
12352111
PubChem Substance ID:
329770253
Beilstein/REAXYS Number:
505995
MDL number:
MFCD00007941
Assay:
≥99.5% (GC/T)
Technique(s):
hybridization: suitable
Bp:
210 °C (lit.)
Vapor pressure:
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
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Quality Level

200

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

grade

ACS reagent, puriss. p.a.

assay

≥99.5% (GC/T)

form

liquid

autoignition temp.

932 °F

expl. lim.

2.7-19 % (lit.)

technique(s)

hybridization: suitable

impurities

≤0.02% free acid (as HCOOH), ≤0.1% water

refractive index

n20/D 1.447

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

transition temp

solidification point 2-3 °C

density

1.134 g/mL at 25 °C (lit.)

cation traces

Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Na: ≤20 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Zn: ≤1 mg/kg

suitability

complies for miscibility

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

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pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

target_organs

Blood

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

305.6 °F

flash_point_c

152 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

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J Casey et al.
Nucleic acids research, 4(5), 1539-1552 (1977-01-01)
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is
The formamide method for the extraction of polysaccharides from haemolytic streptococci.
Fuller AT.
British Journal of Experimental Pathology, 32(1), 130-130 (1960)
Solubilization in formamide protects RNA from degradation.
Chomczynski P.
Nucleic Acids Research, 20(14), 3791-3791 (1992)
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Global Trade Item Number

SKUGTIN
93572-25ML-F04061837675980