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About This Item
NACRES:
NA.31
UNSPSC Code:
41105300
Storage temp.:
2-8°C
packaging
pack of 1 ea
manufacturer/tradename
Cytiva RPN3000
storage temp.
2-8°C
General description
ECL™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Application
ECL™ Direct Nucleic Acid has been used in Western blot, southern blot and hybridization.
Features and Benefits
- Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL™ Direct, Hybond® N+, and Hyperfilm ECL.
- Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
- No need to strip blots before reprobing.
- For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
- Consistent results combining strong signals with very Low backgrounds.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva
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Danger
Storage Class
12 - Non Combustible Liquids
Regulatory Information
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Articles
描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
Kostyantyn V Dmytruk et al.
BMC biotechnology, 11, 58-58 (2011-05-27)
The detection and quantification of uric acid in human physiological fluids is of great importance in the diagnosis and therapy of patients suffering from a range of disorders associated with altered purine metabolism, most notably gout and hyperuricaemia. The fabrication
The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. bolletii.
Leao SC, et al.
PLoS ONE, 8(4), e60746-e60746 (2013)
CRISPR-PCS Protocol for Chromosome Splitting and Splitting Event Detection in Saccharomyces cerevisiae.
Sasano Y and Harashima S
Bio-protocol, 7(10) (2017)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GERPN3000 | 04061834085140 |