Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.31
UNSPSC Code:
41105300
Storage temp.:
2-8°C
packaging
pack of 1 ea
manufacturer/tradename
Cytiva RPN3001
storage temp.
2-8°C
General description
ECL™ Direct Labeling and Detection System.
Application
ECL™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Features and Benefits
- Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL™ Direct, Hybond® N+, and Hyperfilm ECL.
- Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
- No need to strip blots before reprobing.
- For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
- Consistent results combining strong signals with very Low backgrounds.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva
Still not finding the right product?
Explore all of our products under ECL™ Direct Nucleic Acid
signalword
Danger
Storage Class
12 - Non Combustible Liquids
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
C Couturier et al.
Arteriosclerosis, thrombosis, and vascular biology, 20(12), 2559-2565 (2000-12-16)
Type II secreted phospholipase A(2) (sPLA(2)) releases precursors of important inflammatory lipid mediators from phospholipids. Some observations have indicated that the sPLA(2), which has been implicated in chronic inflammatory conditions such as arthritis, contributes to atherosclerosis in the arterial wall.
H M Peng et al.
Molecular pharmacology, 54(4), 740-747 (1998-10-10)
The regulation of cytochrome P450 (CYP) 2E1, the ethanol-inducible isoform, is particularly complex. The level is affected by a variety of other foreign compounds, by insulin (as studied in several laboratories), and by triiodothyronine (T3), which has not been previously
C Couturier et al.
The Journal of biological chemistry, 274(33), 23085-23093 (1999-08-07)
Type II-secreted phospholipase A(2) (type II-sPLA(2)) is expressed in smooth muscle cells during atherosclerosis or in response to interleukin-1beta. The present study shows that the induction of type II-sPLA(2) gene by interleukin-1beta requires activation of the NFkappaB pathway and cytosolic
Global Trade Item Number
| SKU | GTIN |
|---|---|
| GERPN3001 | 04061834085157 |