R4018
Ribonucleic acid, transfer, phenylalanine specific from brewer′s yeast
Synonym(s):
Transfer RNA, Yeast Phe tRNA, tRNA
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About This Item
Form:
lyophilized powder
grade
Molecular Biology
form
lyophilized powder
mol wt
25 kDa
solubility
water: 2 mg/mL
storage temp.
2-8°C
General description
Transfer RNA is isolated from brewer′s yeast. This transfer RNA is specific for transfer of the amino acid phenylalanine.
The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.
The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.
This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.
The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.
The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.
This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.
Application
Suitable for use:
- As a tRNA standard
- As a substrate in tRNA synthatase assays
- As a substrate in other reactions involving tRNAs
Preparation Note
Prepared by method of Wimmer, E., et al., Biochemistry, 7, 2623 (1968).
Other Notes
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Related Content
Mariana Pavon-Eternod et al.
Nucleic acids research, 37(21), 7268-7280 (2009-09-29)
Increased proliferation and elevated levels of protein synthesis are characteristics of transformed and tumor cells. Though components of the translation machinery are often misregulated in cancers, what role tRNA plays in cancer cells has not been explored. We compare genome-wide
Tissue-specific differences in human transfer RNA expression.
Dittmar, K.A., et al.
PLoS Genetics, 2, 2107-2107 (2006)
W Yan et al.
The EMBO journal, 17(16), 4809-4817 (1998-08-26)
Correct folding of newly synthesized polypeptides is thought to be facilitated by Hsp70 molecular chaperones in conjunction with DnaJ cohort proteins. In Saccharomyces cerevisiae, SSB proteins are ribosome-associated Hsp70s which interact with the newly synthesized nascent polypeptide chain. Here we
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R4018-1MG | 04061826683859 |
| R4018-5MG | 04061836695170 |