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Merck
CN

R9001

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

Type X, lyophilized powder

Synonym(s):

Transfer RNA, tRNA

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About This Item

CAS Number:
UNSPSC Code:
41106305
NACRES:
NA.55
MDL number:
Form:
lyophilized powder
Quality level:
Technical Service
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grade

Molecular Biology

Quality Level

type

Type X

form

lyophilized powder

storage temp.

−20°C

General description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation.

Application

Suitable for use as a carrier in nucleic acid purification and precipitation.

Other Notes

tRNA is provided as a lyophilized powder.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品

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Mammalian in vitro splicing assays.
A Mayeda et al.
Methods in molecular biology (Clifton, N.J.), 118, 315-321 (1999-11-05)
Sean P Ryder et al.
Methods in molecular biology (Clifton, N.J.), 488, 99-115 (2008-11-05)
The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to resolve free from bound RNA with high resolution in a gel matrix. We review the quantitative application of this approach to
Christophe Maris et al.
Nucleic acids research, 48(8), 4521-4537 (2020-03-15)
The polypyrimidine tract binding protein (PTB) is a multi-domain protein involved in alternative splicing, mRNA localization, stabilization, polyadenylation and translation initiation from internal ribosome entry sites (IRES). In this latter process, PTB promotes viral translation by interacting extensively with complex



Global Trade Item Number

SKUGTIN
R9001-10KU04061833221198
R9001-2KU04061836824099