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R8508

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

buffered aqueous solution

Synonym(s):

Transfer RNA, tRNA

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About This Item

CAS Number:
UNSPSC Code:
12352106
NACRES:
NA.55
MDL number:
Form:
buffered aqueous solution
Quality level:
Technical Service
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grade

Molecular Biology

Quality Level

form

buffered aqueous solution

concentration

9-11 mg/mL

foreign activity

DNase, Nickase, none detected

shipped in

dry ice

storage temp.

−20°C

General description

Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.
Transfer RNA (tRNA) is isolated from baker′s yeast.

Application

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription. It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation.

Biochem/physiol Actions

Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.

Preparation Note

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Global Trade Item Number

SKUGTIN
R8508-1ML04061838107183
R8508-5X1ML04061838099785