Citrate Buffer, pH 6.0, 10×, Antigen Retriever

Name: Citrate Buffer, pH 6.0, 10×, Antigen Retriever
Brand: SIGMA

for immunohistochemistry

Synonym(s):

1,2,3-Propanetricarboxylic acid, 2-hydroxy-, mixt. with sodium hydroxide (Na(OH)) and sodium 2-hydroxy-1,2,3-propanetricarboxylate

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About This Item

NACRES:

NA.47

UNSPSC Code:

12352107

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Properties

Quality Level

100

form

liquid

pH

6.0

application(s)

hematology
histology

storage temp.

2-8°C

Description

Application

Citrate Buffer, pH 6.0, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Citrate buffer, pH 6.0, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.

Preparation Note

Dilute the Citrate buffer, pH 6.0, 10x, Antigen Retriever 10-fold with water to prepare a 1x Working Solution, e.g., dilute 10 mL of 10x concentrate with 90 mL of water.

Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.

Other Notes

Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer.
Store the prepared 1′ Citrate buffer, pH 6.0, Working Solution at 2–8 °C.