D9905
Denhardt′s Solution
for Northern and Southern blotting, lyophilized powder
Synonym(s):
Denhardt′s Solution For Blotting Technique
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
grade
Molecular Biology
Quality Level
form
lyophilized powder
technique(s)
Southern blotting: suitable
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
2-8°C
General description
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Application
Denhardt′s Solution has been used:
- as a component in hybridization buffer for the hybridization of brain sections
- in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
- in the preparation of hybridization mixture
Suitable as a blocking Buffer for Southern and Northern blot membranes.
Biochem/physiol Actions
Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.
Preparation Note
Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Alterations in m-RNA expression for Cu, Zn-superoxide dismutase and glutathione peroxidase in the basal ganglia of MPTP-treated marmosets and patients with Parkinson?s disease
Kunikowska G and Jenner P
Brain Research, 968(2), 206-218 (2003)
Nancy Stralis-Pavese et al.
Nature protocols, 6(5), 609-624 (2011-04-30)
Microbial diagnostic microarrays (MDMs) are highly parallel hybridization platforms containing multiple sets of immobilized oligonucleotide probes used for parallel detection and identification of many different microorganisms in environmental and clinical samples. Each probe is approximately specific to a given group
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D9905-5X5ML | 04061833591345 |
| D9905-5ML | 04061833591338 |