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L2005

Lactoperoxidase from bovine milk

lyophilized powder (essentially salt-free), ≥200 units/mg protein

Synonym(s):

Donor:hydrogen peroxide oxidoreductase, Peroxidase

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About This Item

CAS Number:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-668-6
MDL number:
EC Number:
Specific activity:
≥200 units/mg protein
Biological source:
bovine milk
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biological source

bovine milk

Quality Level

form

lyophilized powder (essentially salt-free)

specific activity

≥200 units/mg protein

absorbance ratio

A412/280 nm 0.7-0.9

UniProt accession no.

storage temp.

−20°C

SMILES string

[O+H2]O[O-]

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

Gene Information

cow ... LPO(280844)

General description

Lactoperoxidase is a major enzyme present in bovine milk and belongs to the peroxidase family. It is present in both plants and animals. The enzyme structure comprises a single polypeptide chain made up of 612 amino acid residues.

Application

Lactoperoxidase from bovine milk has been used as a standard for milk lactoperoxidase to study the role of H2O2 in the inhibition of Staphylococcus aureus growth by Lactococcus garvieae in the presence of lactoperoxidase in raw milk. It has also been used in lactoperoxidase-mediated iodination to prepare radiolabeled peptides.

Biochem/physiol Actions

Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.
Lactoperoxidase contributes to the antimicrobial system of milk by inactivating a wide range of micro-organisms. This lactoperoxidase-mediated antimicrobial system is also identified in human secretions such as tear-fluid, saliva, and milk. Lactoperoxidase catalyzes the oxidation of molecules by releasing H2O2. The product exhibits antimicrobial activity.

Analysis Note

Protein determined by Lowry method.

Other Notes

One unit will oxidize 1.0 μmole of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 5.5 at 25 °C.


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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S Linde et al.
International journal of peptide and protein research, 15(5), 495-502 (1980-05-01)
Monoiodoinsulin was prepared using ion exchange chromatography. The isolated monoiodoinsulin showed on polyacrylamide gel electrophoresis two bands with different intensities related to the initial method of iodination. Each of the two bands were isolated from the gel, and determination of
Céline Delbes-Paus et al.
Food microbiology, 27(7), 924-932 (2010-08-07)
The response of Staphylococcus aureus growth inhibition by Lactococcus garvieae to catalase and milk lactoperoxidase, and its efficiency in raw milk cheese were evaluated. S. aureus and L. garvieae were co-cultivated in broth buffered at pH 6.8, and in raw
Wen Liu et al.
Biochemical pharmacology, 85(10), 1504-1512 (2013-03-20)
NLRP3 inflammasome has been reported to be associated with various kinds of immunological diseases including colitis. However, there are few drug candidates targeting inflammasomes for the treatment of colitis. In the present study, we aimed at examining the effect of



Global Trade Item Number

SKUGTIN
L2005-10MG04061832730585
L2005-1MG04061826304297
L2005-25MG04061833949665
L2005-5MG04061833949672