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Merck
CN

L3771

Sodium dodecyl sulfate

greener alternative

BioReagent, suitable for electrophoresis, Molecular Biology, ≥98.5% (GC)

Synonym(s):

Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate

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About This Item

Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
Molecular Weight:
288.38
NACRES:
NB.22
PubChem Substance ID:
eCl@ss:
39093306
UNSPSC Code:
12161902
EC Number:
205-788-1
MDL number:
Beilstein/REAXYS Number:
3599286
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biological source

synthetic

Quality Level

grade

Molecular Biology

description

anionic

product line

BioReagent

assay

≥98.5% (GC)

form

powder

mol wt

micellar 18,000

greener alternative product characteristics

Catalysis
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

aggregation number

62

technique(s)

electrophoresis: suitable

impurities

≤2.0% water (Karl Fischer)

CMC

7-10 mM (20-25°C)

mp

204-207 °C (lit.)

transition temp

cloud point >100 °C

solubility

water: soluble

density

1.03 g/cm3 at 20 °C (68 °F)

anion traces

chloride (Cl-): ≤500 ppm, phosphate (PO43-): ≤10 ppm

cation traces

heavy metals (as Pb): ≤10 ppm

absorption

≤0.1 at 260 at 3%, ≤0.1 at 280 at 3%

HLB

40

suitability

suitable for electrophoresis

foreign activity

DNase, RNase, none detected

greener alternative category

storage temp.

room temp

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

General description

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is an anionic surfactant widely used in various scientific applications, particularly in molecular biology, biochemical, and cell biology research. Its effectiveness stems from its amphiphilic nature, allowing it to interact with both hydrophobic and hydrophilic molecules. SDS is a versatile detergent that excels in solubilizing and denaturing proteins, making it an essential tool in protein extraction and purification procedures. By disrupting protein structures, SDS facilitates the separation of proteins based on their molecular weight during SDS-PAGE electrophoresis.

Beyond protein analysis, SDS also finds applications in the electrophoretic separation of lipids, enabling the study and characterization of these important cellular components. Additionally, SDS is employed in various hybridization and molecular biology techniques, demonstrating its versatility across a range of research areas. SDS′s effectiveness in both ionic and anionic conditions further expands its utility, allowing it to adapt to diverse experimental setups and requirements. Its ability to unravel proteins by binding to peptide chains and imparting a negative charge effectively eliminates protein shape as a factor for gel separation, ensuring accurate protein size determination.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is used as a surfactant to enhance catalysis.  Click here for more information.

Application

Anionic detergent
Sodium dodecyl sulfate has been used:
  • in chromatin immunoprecipitation
  • in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
  • as a component of radioimmunoprecipitation assay buffer for immunoblotting
Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

Features and Benefits

  • Highly versatile surfactant for your molecular biology and biochemical research
  • Suitable for electrophoresis applications
  • Tested to confirm low levels of heavy metal contamination,ensuring suitability for various applications.

Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.

Other Notes

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

4.1B - Flammable solid hazardous materials

wgk

WGK 2

flash_point_f

338.0 °F

flash_point_c

170 °C

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges



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Protocols

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

本页内容介绍和讨论了三种免疫印迹膜剥离和重新检测的方案。

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

View All Protocols

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Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.


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Global Trade Item Number

SKUGTIN
L3771-1KG04061835546039
L3771-100G04061835546022
L3771-10KG04061841231974
L3771-25G04061835546046
L3771-500G04061835546053