Anti-Nanog antibody, Mouse monoclonal

Anti-Nanog antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma NNG-811 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human Nanog. The human Nanog gene product is a 305 amino acid, 35 kDa protein with a tryptophan repeat domain and two C-terminal trans-activating domains. It is primarily located in the nucleus.

Nanog is a homeodomain transcription factor expressed in cells of the inner cell mass of early embryos, in embryonic stem cells (ESC), germ cells and in stem/progenitor cells of some tissues. Together with Oct-4, Sox-2, and Rex-1, it is a molecular marker for pluripotent cells and for undifferentiated stem cells. This protein is crucial for the maintenance of pluripotency of ESCs and is down-regulated when ESCs differentiate.
Nanog controls the expression of many ESC genes together with other stem cell transcription factors like Oct-4 and Sox-2. Nanog targets both repressor and activator complexes to regulatory regions of hundreds of genes in the genome. Expression of nanog can be detected primarily in germ cell tumors and in tumors of other cell types. Nanog is an important marker for Seminomas, testicular carcinomas, teratocarcinomas, and germ cell-like tumors in various tissues. Furthermore, it was shown to transform NIH3T3 cells.

recombinant human Nanog.

Anti-Nanog antibody, Mouse monoclonal has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• flow cytometric analysis
• immunoprecipitation
• immunofluorescence

Monoclonal Anti-Nanog antibody is suitable for use in ELISA, immunoblotting (~ 40 kDa), immunocytochemistry and immunoprecipitation.
Immunoblotting: a working antibody concentration of 2-4 mg/mL is recommended using extracts of NT2 cells.

Monoclonal Anti-Nanog reacts specifically with human Nanog.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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