S2015
SSPE Buffer 20× Concentrate
for Northern and Southern blotting, solution
Synonym(s):
20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer
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About This Item
NACRES:
NA.25
UNSPSC Code:
41105319
grade
Molecular Biology
Quality Level
sterility
0.2 μm filtered
form
solution
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.
Application
Suitable for:
- Hybridization buffer for Northern and Southern blots
- Washing buffer for microarrays
Features and Benefits
- Convenient ready-to-use liquid
- Multi-purpose use in hybridizations
Preparation Note
SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Glucocorticoid antagonist RU38486 fails to block acid-induced muscle wasting in vivo or in vitro.
Pickering WP, et al.
Nephrology, Dialysis, and Transplantation, 18, 1475-1484 (2003)
Warren P Pickering et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 18(8), 1475-1484 (2003-08-05)
Increased protein degradation during metabolic acidosis contributes to muscle wasting in uraemia. Adrenalectomy experiments in severely acidotic rats (arterial pH approximately 7.15) have shown that this is prevented in the absence of glucocorticoid. It should therefore be possible to block
Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures.
Pierce SE, et al.
Nature Protocols, 2, 2958-2974 (2007)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S2015-1L | 04061836916527 |