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S8140

SSPE Buffer

Name: SSPE Buffer
Brand: SIGMA

for Northern and Southern blotting, powder blend

Synonym(s):

Saline-Sodium Phosphate-EDTA Buffer

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About This Item

NACRES:

NA.25

UNSPSC Code:

41105319

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Properties

grade

Molecular Biology

Quality Level

200

form

powder blend

pH

7.2-7.6 (20 × in water, high purity, ambient)

solubility

water, high purity: 20 ×, clear, colorless

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

Description

General description

SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.

Application

Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).

Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).

Features and Benefits

Easy to reconstitute powder
Multi-purpose use in hybridizations

Preparation Note

Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Protocols

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Microfluidic-based enzymatic on-chip labeling of miRNAs

Sonja Vorwek

New Biotechnology (2008)

3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

Igor V. Kutyavin

Nucleic Acids Research, 28(2) (2000)

Global Trade Item Number

SKU	GTIN
S8140-1L	04061838061102
S8140-100ML	04061835509591