Skip to Content
Merck
CN

Key Documents

View All Documentation

T7513

Thrombin from bovine plasma

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

Synonym(s):

Factor IIa

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

CAS Number:
9002-04-4
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
MFCD00166053
EC Number:
3.4.21.5 (BRENDA, IUBMB)
Specific activity:
≥2,000 NIH units/mg protein (E1%/280 = 19.5)
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

lyophilized powder

Quality Level

200

specific activity

≥2,000 NIH units/mg protein (E1%/280 = 19.5)

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

UniProt accession no.

P00735

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

cow ... F2(280685)

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Still not finding the right product?

Explore all of our products under Thrombin from bovine plasma

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCL5520
SLCK6302
SLCJ7952
SLCJ0343
SLCH4980

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

牛血浆凝血酶

凝血酶是一种内溶性丝氨酸蛋白酶，可选择性裂解纤维蛋白原的精氨酸-甘氨酸键，从而形成纤维蛋白并释放纤维蛋白肽A和B。

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

Articles

凝血酶因子IIa

凝血酶因子IIa是一种内切丝氨酸蛋白酶，可选择性地裂解纤维蛋白原的Arg -Gly键，形成纤维蛋白并释放纤维蛋白肽A和B。

Thrombin Factor IIa

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

Related Content

J Y Chueh et al.
AJNR. American journal of neuroradiology, 32(7), 1237-1244 (2011-05-21)
Mechanical behavior of the thromboembolus is one of the key factors that determine the efficacy of thrombectomy devices for revascularization in AIS. We characterized the mechanical properties and composition of thromboemboli from clinical cases and compared them with commonly used
U Seligsohn
Haemophilia : the official journal of the World Federation of Hemophilia, 18 Suppl 4, 161-165 (2012-06-29)
For patients affected by severe inherited platelet dysfunctions, e.g. Glanzmann thrombasthenia (GT) or Bernard-Soulier syndrome (BSS), platelet transfusion is frequently needed for controlling spontaneous bleeding, and is always needed when trauma occurs or surgery is performed. For the mild-to-moderate bleeding
B Bijina et al.
Saudi journal of biological sciences, 18(3), 273-281 (2011-07-01)
Protease inhibitors are well known to have several applications in medicine and biotechnology. Several plant sources are known to return potential protease inhibitors. In this study plants belonging to different families of Leguminosae, Malvaceae, Rutaceae, Graminae and Moringaceae were screened
View All Related Papers

Global Trade Item Number

SKUGTIN
T7513-250UN04061835559350
T7513-500UN04061835559367
T7513-1000KU04061831169485
T7513-100UN04061835546435
T7513-50UN04061837373510