Y1771
Yeast Synthetic Drop-out Medium Supplements
without uracil, leucine, and tryptophan
Synonym(s):
Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements
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About This Item
Quality Level
form
powder
technique(s)
transformation: suitable
application(s)
microbiology
storage temp.
room temp
Application
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:
- the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
- Yarrowia lipolytica PO1f strain positive transformants
- CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants
Preparation Note
Dissolve 1.46 g of supplement per liter of medium.
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
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signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.
Related Content
Instructions
Paul Swietalski et al.
Microbial cell factories, 19(1), 100-100 (2020-05-13)
The secretory production of recombinant proteins in yeast simplifies isolation and purification but also faces possible complications due to the complexity of the secretory pathway. Therefore, correct folding, maturation and intracellular transport of the recombinant proteins are important processing steps
Global Trade Item Number
| SKU | GTIN |
|---|---|
| Y1771-20G | 04061837587979 |