Y2627
2xYT Medium
EZMix Powder microbial growth medium
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
grade
Molecular Biology
Quality Level
description
nutrient rich, quick dissolve, dust free formulation
sterility
non-sterile
form
powder
composition
Inert binder (EZMix only), 0.6 g/L , NaCl, 5 g/L , Tryptone (pancreatic digest of casein), 16 g/L , Yeast extract, 10 g/L
application(s)
agriculture
food and beverages
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
2xYT EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- Easy scale-up using larger package size
- Standard formulation
Preparation Note
Stir to suspend 31.6 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Protocols
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
Related Content
Shuang Yang et al.
eLife, 9 (2020-06-09)
Dynamic trafficking of G protein-coupled receptors (GPCRs) out of cilia is mediated by the BBSome. In concert with its membrane recruitment factor, the small GTPase ARL6/BBS3, the BBSome ferries GPCRs across the transition zone, a diffusion barrier at the base