Quality Level
form
liquid
usage
sufficient for 100 reactions, sufficient for 2000 reactions, sufficient for 500 reactions
feature
dNTPs included, hotstart
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
colorless
input
purified DNA
suitability
suitable for (quantitative PCR)
application(s)
agriculture
compatibility
Bio-Rad MyiQ ( ), Bio-Rad iCycler iQ, Bio-Rad iQ 5, for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7500 Fast, for use with ABI 7500, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI 7900, for use with ABI StepOne, for use with ABI StepOnePlus, for use with ABI ViiA 7, for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MJ Opticon, for use with Bio-Rad MiniOpticon, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Eppendorf® Mastercycler ep realplex, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000
detection method
probe-based
shipped in
wet ice
storage temp.
−20°C
General description
- Kit is designed to perform probe-based qPCR assays on commonly available real-time instrument platforms.
- ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays.
- Kit is not compatible with qPCR instruments that utilize glass capillary tubes.
Application
Features and Benefits
- Assay results in as little as 25 minutes
- Designed to deliver unsurpassed accuracy, precision, and sensitivity
- Extensive design and validation of ReadyMix chemistry virtually eliminates the need to optimize assay parameters
- Ideal for rapid, high-throughput 2-step qPCR protocols
- The master mix allows consistency and reproducibility from one reaction to the next
- Reduced preparation time and the risk of contamination from multiple pipetting steps
- Compatible with commonly available real-time instrument platforms for probe-based qPCR assays
- Require small amplicons (<200 bp) for optimal results
- Compatible with commercial primer sets, including TaqMan® assays
- JumpStart™ Taq DNA polymerase allows room temperature set-up and the hot-start mechanism prevents primer-dimer and non-specific product formation
Packaging
100x ROX internal reference dye, Catalog Number R4526. Optional, for use with machines compatible with an internal reference dye, e.g., ABI and Stratagene.
Default reaction volume is 50 μL
100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL
Other Notes
- Commercially available 5′ nuclease assays (e.g., TaqMan® Assays) are compatible with LuminoCt ReadyMix.
- At a minimum, use software to design primers and probes for assays and ensure that qPCR amplicons are <200 bp.
- Sigma recommends designing primers and probes with Beacon Designer software
To order primers and probes from Sigma, visit www.sigma.com/oligos
Legal Information
存储类别
10 - Combustible liquids
wgk
WGK 1
法规信息
此项目有
实验方案
我们的 SYBR Green qPCR 协议是专为检测基因表达和 RT-PCR 反应的精确定量而设计的方法。
The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.
qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.
商品
聚合酶链反应是分子生物学应用最普遍的技术之一。PCR过程主要由三步组成：变性、退火和延伸
qPCR探针利用荧光标记的靶特异性探针，从而提高了特异性和灵敏度。此外，还有多种荧光染料可供选择，因此可以使用多个引物同时扩增多个序列。
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L6669-100RXN | 04061835574308 |
| L6669-2000RXN | 04061838043832 |
| L6669-500RXN | 04061833959008 |