P4439
碱性磷酸酶 来源于猪肾脏
lyophilized powder, ≥100 DEA units/mg protein
别名:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
Specific activity:
≥100 DEA units/mg protein
form
lyophilized powder
Quality Level
specific activity
≥100 DEA units/mg protein
composition
Protein, ≥50%
storage temp.
−20°C
Application
碱性磷酸酶可与抗体和其他蛋白质偶联，用于ELISA、蛋白质免疫印迹和组织化学检测。 碱性磷酸酶也可用于防止DNA自身连接和放射性标记。
Biochem/physiol Actions
碱性磷酸酶是一种水解酶，能使核苷酸、蛋白质和生物碱等分子去磷酸化。
Packaging
包装规格取决于DEA单位
Physical form
从Tris-HCl缓冲液中冻干，含氯化镁和氯化锌，pH 8.0
Analysis Note
通过缩二脲法测定的蛋白质。
Other Notes
在pH 9.8和37℃条件下，1.0个单元每分钟水解1 μmol对硝基苯基磷酸盐。
hcodes
signalword
Danger
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
Sequencing end-labeled DNA with base-specific chemical cleavages.
A M Maxam et al.
Methods in enzymology, 65(1), 499-560 (1980-01-01)
Insulin affects dopamine metabolism in Drosophila females under normal and stress conditions.
N V Adonyeva et al.
Doklady. Biochemistry and biophysics, 448, 40-42 (2013-03-13)
C Cavallo et al.
Journal of biological regulators and homeostatic agents, 27(1), 165-175 (2013-03-16)
Bone marrow is one of the best characterized stem cell microenvironments that contains Mesenchymal Stem Cells (MSCs), a rare population of non-hematopoietic stromal cells. MSCs have been indicated as a new option for regenerative medicine because of their ability to
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P4439-100UN | 04061834374602 |