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Merck
CN

T5574

TB培养基

Liquid microbial growth medium

别名:

TB 培养基, Tartoff – Hobbs 培养基

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关于此项目

NACRES:
NA.85
UNSPSC Code:
41106200
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grade

Molecular Biology

Quality Level

description

Liquid Medium

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

Terrific Broth是一个被高度引用的用于大肠杆菌培养的微生物生长培养基。 这种营养丰富的微生物肉汤含有甘油、肽、氨基酸、水溶性维生素和碳水化合物。

Application

Terrific肉汤用于非选择性培养大肠杆菌菌株,用于克隆、高产量生产DNA质粒和高产量生产重组蛋白。引用 它也适用于添加适当抗生素时的选择性培养。

Features and Benefits

Terrific Broth液可提供:
  • 过滤器灭菌,即用型
  • 方便包装规格
  • 标准配方

Other Notes

23.6g/L酵母提取物
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L甘油


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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

动物来源培养基

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

General protocols for growth of competent cells in microbial medium.

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.


The preparation and analysis of DNA for use in transgenic technology.
Auerbach, A.B., et al.
Advanced Protocols For Animal Transgenesis, 501-501 (2011)
Andrey Sokolov et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(3), 969-974 (2010-01-19)
Whereas the laws of thermodynamics prohibit extraction of useful work from the Brownian motion of particles in equilibrium, these motions can be "rectified" under nonequilibrium conditions, for example, in the presence of asymmetric geometrical obstacles. Here, we describe a class
Shani Leviatan et al.
The Journal of biological chemistry, 285(31), 23548-23556 (2010-06-08)
Membrane proteins constitute 20-30% of all proteins encoded by the genome of various organisms. Large amounts of purified proteins are required for activity and crystallization attempts. Thus, there is an unmet need for a heterologous membrane protein overexpression system for



全球贸易项目编号

货号GTIN
T5574-500ML04061837347993
CR40057-1000M04061838445797