grade
Molecular Biology
Quality Level
description
Liquid Medium
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Terrific Broth是一个被高度引用的用于大肠杆菌培养的微生物生长培养基。 这种营养丰富的微生物肉汤含有甘油、肽、氨基酸、水溶性维生素和碳水化合物。
Application
Terrific肉汤用于非选择性培养大肠杆菌菌株,用于克隆、高产量生产DNA质粒和高产量生产重组蛋白。引用 它也适用于添加适当抗生素时的选择性培养。
Features and Benefits
Terrific Broth液可提供:
- 过滤器灭菌,即用型
- 方便包装规格
- 标准配方
Other Notes
23.6g/L酵母提取物
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L甘油
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L甘油
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
动物来源培养基
此项目有
实验方案
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
The preparation and analysis of DNA for use in transgenic technology.
Auerbach, A.B., et al.
Advanced Protocols For Animal Transgenesis, 501-501 (2011)
Andrey Sokolov et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(3), 969-974 (2010-01-19)
Whereas the laws of thermodynamics prohibit extraction of useful work from the Brownian motion of particles in equilibrium, these motions can be "rectified" under nonequilibrium conditions, for example, in the presence of asymmetric geometrical obstacles. Here, we describe a class
Shani Leviatan et al.
The Journal of biological chemistry, 285(31), 23548-23556 (2010-06-08)
Membrane proteins constitute 20-30% of all proteins encoded by the genome of various organisms. Large amounts of purified proteins are required for activity and crystallization attempts. Thus, there is an unmet need for a heterologous membrane protein overexpression system for
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T5574-500ML | 04061837347993 |
| CR40057-1000M | 04061838445797 |