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B2059

Biotin Polyethyleneoxide Iodoacetamide

Name: Biotin Polyethyleneoxide Iodoacetamide
Brand: SIGMA

Synonym(s):

Biotin PEO Iodoacetamide

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About This Item

Empirical Formula (Hill Notation):

C₁₈H₃₁IN₄O₅S

Molecular Weight:

542.43

NACRES:

NA.32

PubChem Substance ID:

24891627

UNSPSC Code:

12352119

MDL number:

MFCD04974830

Form:

powder

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Properties

description

Sulfhydryl specific, water soluble biotinylation reagent

Quality Level

200

form

powder

solubility

H₂O: 10 mg/mL

storage temp.

2-8°C

SMILES string

ICC(=O)NCCOCCOCCNC(=O)CCCCC1SCC2NC(=O)NC12

InChI

1S/C18H31IN4O5S/c19-11-16(25)21-6-8-28-10-9-27-7-5-20-15(24)4-2-1-3-14-17-13(12-29-14)22-18(26)23-17/h13-14,17H,1-12H2,(H,20,24)(H,21,25)(H2,22,23,26)

InChI key

LWGLKVINXNMNJX-UHFFFAOYSA-N

Description

General description

Biotin polyethyleneoxide (PEO) iodoacetamide is a sulfhydryl (thiol) specific biotinylation reagent.

Application

Biotin Polyethyleneoxide Iodoacetamide has been used as a thiol reactive reagent to demonstrate the presence of free thiols in capsid protein L1 of human papillomavirus 16. It has been used for biotinylation of proteins for ELISA (enzyme-linked immunosorbent assay) competition and pull down experiments.

Incorporates a 12 atom hydrophilic spacer.
Typically coupled to sulfhydryl groups at pH 7.5 - 8.5
Especially useful for cysteine labeling in many proteomics type applications such as peptide mapping and mass spectrometry.

Biochem/physiol Actions

Biotin polyethyleneoxide (PEO) iodoacetamide group reacts specifically with reduced thiols at pH 7.5 - 8.5. Peptides and small molecules containing thiol groups may also be labeled using this reagent. The biotin PEO iodoacetamide enables tagging of cysteine residues and conjugation to sulfhydryls in proteins making it useful in proteomics, peptide mapping, phosphopeptide analysis, and mass spectrometry.

pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Global Trade Item Number

SKU	GTIN
B2059-50MG	04061833288955