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G4142

β-Galactosidase from bovine testes

ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)

Synonym(s):

β-D-Galactoside galactohydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
EC Number:
Specific activity:
1.0-3.0 units/mg protein (modified Warburg-Christian)
Biological source:
bovine
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biological source

bovine

Quality Level

form

ammonium sulfate suspension

specific activity

1.0-3.0 units/mg protein (modified Warburg-Christian)

foreign activity

α- and β-glucosidase ≤0.2%, α-fucosidase ≤0.2%, α-galactosidase ≤0.2%, α-mannosidase ≤0.2%, β-N-acetylglucosaminidase ≤1.0%

storage temp.

2-8°C

Application

β-Galactosidase from bovine testes was used for coupling to Sepharose.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH approx. 5.0

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

低风险生物材料

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Articles

以硫酸铵悬浮液形式提供的酶的使用指南

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Related Content


W Kiess et al.
The Journal of biological chemistry, 263(19), 9339-9344 (1988-07-05)
Cloning and sequencing of the human type II insulin-like growth factor (IGF) receptor cDNA revealed an 80% deduced amino acid sequence homology with the bovine cation-independent mannose 6-phosphate (Man-6-P) receptor, suggesting identity of the two receptors (Morgan, D. O., Edman
The purification and properties of beta-galactosidase from bovine testes.
J J Distler et al.
The Journal of biological chemistry, 248(19), 6772-6780 (1973-10-10)
Screening of high-risk Gaucher disease patients using dried blood spots techniques.
Filippo Vairo et al.
Gene, 523(1), 114-115 (2013-04-10)



Global Trade Item Number

SKUGTIN
G4142-.2UN04061832843766
G4142-.5UN04061832843773