T6424
Trypsin from human pancreas
salt-free, lyophilized powder, vial of ≥1,000 BAEE units
Synonym(s):
Serine Protease 1
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-650-8
MDL number:
Biological source:
human pancreas
biological source
human pancreas
Quality Level
form
salt-free, lyophilized powder
packaging
vial of ≥1,000 BAEE units
storage temp.
−20°C
Gene Information
human ... PRSS1(5644), PRSS2(5645), PRSS3(5646)
General description
Trypsin is a serine protease. The pancreas produce the precursor trypsinogen and secretes it to the intestine. Trypsinogen is converted to trypsin by enterokinase in the intestinal mucous membrane.
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Application
Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells.
Preparation Note
Minimum 1,000 BAEE units/vial
Analysis Note
Protein determined by E1%/280.
Other Notes
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Regulatory Information
高风险级别生物产品--人源产品
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Protocols
本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
Industrial Enzymes and Their Applications (1998)
Kelly A Dryden et al.
Journal of molecular biology, 422(5), 650-658 (2012-06-30)
Human astroviruses (HAstVs) are a major cause of gastroenteritis. HAstV assembles from the structural protein VP90 and undergoes a cascade of proteolytic cleavages. Cleavage to VP70 is required for release of immature particles from cells, and subsequent cleavage by trypsin
Jia Liu et al.
Nature protocols, 16(7), 3522-3546 (2021-06-06)
Cellular heterogeneity is pervasive and of paramount importance in biology. Single-cell analysis techniques are indispensable for understanding the heterogeneity and functions of cells. Low-copy-number proteins (fewer than 1,000 molecules per cell) perform multiple crucial functions such as gene expression, cellular
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T6424-1VL | 04061837371493 |