Quality Level
form
powder blend
pH
6.8-7.2 (20-25 °C, 263 mg/L in water)
solubility
water: 263 mg/mL, clear, colorless
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSC缓冲液20x粉末是Southern和Northern blotting的一种标准试剂。该浓缩后的粉末应在使用前用分子生物学级水重构至指定的最终体积（终溶液为20x）。该浓缩液可直接使用，或稀释后制成浓度低至0.5x的SSC工作溶液。
Application
柠檬酸钠盐（SSC）缓冲液在微阵列技术中已被用作洗涤液。它也被用作荧光原位杂交（FISH）缓冲液中的一种组分。
适用于：
- 制备用于杂交的核酸
- 制备用于Northern和Southern印迹的杂交缓冲液
- 制备用于Northern和Southern印迹的洗涤液
Features and Benefits
- 在杂交中具有多种用途
- 可简单的被重构并稀释成任何所需的浓度
Preparation Note
20x的SSC缓冲液终浓缩液在3M NaCl中含有0.3 M柠檬酸钠。
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存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization
Bolanos V, et al.
The Plant Journal (2013)
AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects
Papp D, et al.
Autophagy, 12(2), 273-286 (2016)
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S0902-1L | 04061836830960 |
| S0902-5X1L | 04061838175182 |