grade
Molecular Biology
description
Saline-Sodium Citrate buffer, made with ultrapure water
sterility
0.2 μm filtered
form
solution
color
colorless
pH
6.9 —7.1
application(s)
diagnostic assay manufacturing
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSC（柠檬酸钠盐）缓冲液20倍浓缩液是Southern和Northern杂交过程的标准试剂。 它可用于严格控制杂交后洗涤步骤所用洗涤缓冲液。
Application
SSC缓冲液20倍浓缩液已用于
- 用作原位杂交人特异性重复Alu序列的杂交缓冲液
- 用作Southern杂交、小鼠基因组DNA斑点印迹和microRNA的Northern印迹的印迹膜洗涤缓冲液
- 用作L1反转录转座子荧光原位杂交(FISH)的孵育缓冲液
Features and Benefits
- 方便的即用型液体
- 在杂交中具有多种用途
Preparation Note
SSC缓冲液20倍浓缩液由3M氯化钠、0.3M柠檬酸钠组成（pH7.0）。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
商品
可用的荧光原位杂交（FISH）过程、试剂和设备。
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S6639-1L | 04061838258816 |